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Proteins size exclusion chromatograph

Figure 3. The size-exclusion chromatographic separation of a standard protein mixture on a Hibar RT LiChrospher 500 DIOL column. COhimn size, 250 X 4 mm (2 columns) eluant, 50 mM H3PO4 adjusted to pH 7.0 with 2.0 M NaOH -r 1% SDS flow-rate, 0.2 ml/min pressure, 15 kg/cm chart speed, 2.5 mnVntin detection, UV 208 nraO.08 AUFS. Samples, (1) albumin (bovine serum = 68 (KK)) (2) chymo-trypsinogen A (25 000) (3) cytochrome c (12 500) (4) alanine (89). Figure 3. The size-exclusion chromatographic separation of a standard protein mixture on a Hibar RT LiChrospher 500 DIOL column. COhimn size, 250 X 4 mm (2 columns) eluant, 50 mM H3PO4 adjusted to pH 7.0 with 2.0 M NaOH -r 1% SDS flow-rate, 0.2 ml/min pressure, 15 kg/cm chart speed, 2.5 mnVntin detection, UV 208 nraO.08 AUFS. Samples, (1) albumin (bovine serum = 68 (KK)) (2) chymo-trypsinogen A (25 000) (3) cytochrome c (12 500) (4) alanine (89).
In which the ratio m/n is close to 3. The silane was produced by free radical copolymerization of vinyltriethoxysilane with N-vinylpyrrolidone. Its number-average molecular weight evaluated by vapour-phase osmometry was 3500. Porous silica microballs with a mean pore diameter of 225 A, a specific surface area (Ssp) of 130 m2/g and a pore volume of 0.8 cm3/g were modified by the silane dissolved in dry toluene. After washings and drying, 0.55% by weight of nitrogen and 4.65% of carbon remained on the microballs. Chromatographic tests carried out with a series of proteins have proved the size-exclusion mechanism of their separation. [Pg.148]

Lau, S. Y. M., Taneja, A. K., and Hodges, R. S., Effects of high-performance liquid chromatographic solvents and hydrophobic matrices on the secondary and quaternary structure of a model protein. Reversed-phase and size exclusion high-performance liquid chromatography, /. Chromatogr., 317, 129, 1984. [Pg.197]

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See also in sourсe #XX -- [ Pg.268 ]



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Size-exclusion

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