Proteins in proteomics

These considerations about protein composition and structure are helpful for understanding key properties and attributes of proteins in proteomic analysis. The isoelectric point (pi) and the molecular mass are two important properties used in... 

Isobaric tags for relative and absolute quantitation of proteins in proteomic research Janus kinase c-Jun iV-terminal kinase... 

As mentioned, the method of 2D gel uses the power of separation of proteins by resolution based on charge and mass of the proteins in two separate dimensions. A separation based on this dual property of proteins provides high resolution by avoiding the presence of several proteins per band and or cross-contamination of a protein band by other protein components and by visualizing the proteins that occur in a small amount in the cell or body fluid. To attain high resolution of thousands of proteins in proteomics, it is important to start with a protein sample that includes all proteins present in a cell or body fluid. Attention must be paid to include all membrane... 

Proteolysis is the process of cleaving protein molecules into smaller pieces (peptides) via chemical or enz)miatic reactions. Living systems carry out proteolysis to obtain amino acids from protein substrates or as part of the construction of new proteins. In proteomic analyses, it is often... 

Geveart K, Vandekerckhove J Protein identification methods in proteomics. Electrophoresis 2000 21 1145. 

Proteome The entire collection of expressed proteins in an organism. 

The second step in 2D electrophoresis is to separate proteins based on molecular weight using SDS-PAGE. Individual proteins are then visualized by Coomassie or silver staining techniques or by autoradiography. Because 2D gel electrophoresis separate proteins based on independent physical characteristics, it is a powerful means to resolve complex mixtures proteins (Fig. 2.1). Modem large-gel formats are reproducible and are the most common method for protein separation in proteomic studies. 

Proteomics is an interdisciplinary science that includes biology, bioinformatics, and protein chemistry. The purpose of this book is to provide the reader with an overview of the types of questions being addressed in proteomics studies and the technologies used to address those questions. The first chapter is a concise outline of the field as it presently stands. The second chapter provides an overview of the use of 2D-gel electrophoresis and mass spectrometry to identify proteins, as well as post-translational... 

Melton, L. Protein arrays proteomics in multiplex. Nature 2004,429,101-107. 

---