Protein separation, amphoteric gels

In practice, the aqueous solution of proteins to be separated is injected by a syringe into the central section of the polyampholyte gel specimen. Then, the DC electric field is switched on, which induces the formation of a hnear pH gradient along the gel sample. In its turn, the generated pH gradient promotes the migration of protein molecules until they are gradually locahzed at their isoelectric pH. Protein molecules will be separated and concentrated if the isoelectric pH of the proteins coincides with appropriate pH zones of the amphoteric gel. 

Possible application of amphoteric gels for protein separation and purification purpose... 

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