Protein isoelectronic point

A simple way to understand the importance of maintaining the pH of our blood within a narrow range is to look at the pAa of the amino acids that constitute the proteins. The ionic state of the protein is maintained by this value of pAig relative to the pH of the surrounding fluid. As an amino acid usually has two or three pAig values, it is often more useful to discuss it in terms of the isoelectronic point, denoted by p/, which is the pH at which the amino acid is neutral, i.e. the zwitterion form is dominant if the amino acid is stable, and the amino acid does not migrate in... 

IEF is similar in concept to conventional gel IEF a stable pH gradient is formed in the capillary using carrier ampholytes, and proteins are focused in the gradient at their pis. The major difference in performing IEF in the capillary format rather than slab gel is the requirement for mobilizing focused protein zones past the detection point. IEF is described in Capillary Isoelectronic Focusing. ... 

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