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Protein import, nucleus extracts

Fig. 2 Uptake of nuclear proteins by an in virro-assembled nucleus. Permeabilized Xenopus sperm were incubated with an egg extract containing a GFP-NLS fusion protein for 30 min. Samples were stained with DAPl (A) or examined for GFP fluorescence (B). The fully assembled nucleus can import GFP-NLS whereas an assembly intermediate cannot. Scale bar, 10 pm. Fig. 2 Uptake of nuclear proteins by an in virro-assembled nucleus. Permeabilized Xenopus sperm were incubated with an egg extract containing a GFP-NLS fusion protein for 30 min. Samples were stained with DAPl (A) or examined for GFP fluorescence (B). The fully assembled nucleus can import GFP-NLS whereas an assembly intermediate cannot. Scale bar, 10 pm.
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See also in sourсe #XX -- [ Pg.534 ]



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