Protein grouping into color classes

Figure 2. Grouping of proteins into color classes by multi-spectral image analysis. A liver extract was made in iso-urea sample buffer (20) and serial dilutions made from 1/16 to 1/1024. Gels were run on each sample dilution and the linear range was separately determined for blue, brown, red and yellow spots (see Figure 3). Spots from each color class were selected from the linear range and the intensity level from blue, green and red channels of a multispectral scan were used to calculate ratios as previously described...

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