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Protein fragmentation online digestion

Once the identity of phosphopeptides in a digestion mixture is revealed, it is imperative to recognize which amino acid residue (Ser, Thr, or Tyr) is phospho-rylated. This task is accomplished by determining the amino acid sequence of peptide fragments in the protein digest after their fractionation into individual components by RP-HPLC, either off- or online with tandem mass spectrometry [34], The tandem MS methods described below have proved to be practical for this purpose. [Pg.361]

See other pages where Protein fragmentation online digestion is mentioned: [Pg.864]    [Pg.385]    [Pg.243]    [Pg.177]    [Pg.649]    [Pg.366]    [Pg.321]    [Pg.147]    [Pg.67]    [Pg.397]    [Pg.466]    [Pg.487]    [Pg.257]    [Pg.2021]    [Pg.302]    [Pg.308]    [Pg.390]    [Pg.3561]    [Pg.311]    [Pg.2497]   
See also in sourсe #XX -- [ Pg.26 ]



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Protein digestibility

Protein digests

Protein fragmentation

Protein fragments

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