Protein Fragmentation Cyanogen Bromide

Fig. 24.8 Release of somatostatin from a hybrid protein by cyanogen bromide cleavage. Somatostatin can be purified free of cyanogen bromide and fragments of /J-galactosidase.

In another application, not employing column chromatography, it was possible to cleave MDPF-labeled proteins with cyanogen bromide and then resolve the fluorescent fragments by SDS-polyacrylamide gel electrophoresis (Chen-Kiang et ai, 1979) the MDPF proteins had themselves been prepared... 

The primary structure of a polypeptide or protein can be determined directly by fragmenting the protein using cyanogen bromide and enzymes, followed by amino acid sequencing of the resulting fragments. 

Another approach has been the preparation of fragments of cytochrome c, in which the heme group is bound to a portion of the protein, either by tryptic digestion or cyanogen bromide cleavage. 

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