Protein chromatography groups

Now that you understand the basis for the interactions between functional groups in water, you also understand the basis for most interactions DNA-DNA, DNA-RNA, DNA-protein, RNA-protein, protein-protein, protein-ligand, enzyme-substrate (Get the picture ), antibody-antigen, protein-chromatography column—it s all the same stuff. 

The most useful information on the interactions of proteins with surfaces will come from studies analogous to those of protein chromatography, where well-characterized and understood proteins are studied with well-characterized surfaces of known functional group type and density. The information obtained is then analyz-able in such a way as to deduce interaction site densities and interaction energies. Only with such data in hand will we be able to begin to quantitatively treat and understand protein adsorption. 

Separation methods based on size include size exclusion chromatography, ultra-filtration, and ultracentrifugation (see Chapter Appendix). The ionic properties of peptides and proteins are determined principally by their complement of amino acid side chains. Furthermore, the ionization of these groups is pH-dependent. 

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