Protein-binding studies

Aniline, phenols, nitrobenzenes Direct immersion PA (85) GC-MS Protein binding study, determination of free concentrations Vaes et al., 1996 (8)... 

Phosphates and phosphonates Protein binding studies, calcium in sewage water, mineral water, blood serum, biological fluid 131... 

A Shibukawa, Y Kuroda, T Nakagawa. High-performance frontal analysis for drug-protein binding study. J. Pharm. Biomed. Anal. 18(6) 1047-1055 (1999). 

J Oravcova, B Bohs, W Lindner. Drug-protein binding studies. New trends in analytical and experimental methodology. J Chromatogr B 677 1—28, 1996. 

A Shibukawa. Development of high-performance frontal analysis and application to drug-plasma protein binding study. Yakugaku Zasshi 118 554-565, 1998 (in Japanese). 

Nakashima, M., Takeuchi, N., Hamada, M., Matsuyama, K., Ichikawa, M. and Goto, S. (1994) In vivo microdialysis for pharmacokinetic investigations a plasma protein binding study of valproate in rabbits. Biolo ccd el Pharmaceutical Bulletin, 17, 1630-1634. 

Polec-Pawlak K, Abramski JK, Semenova O, Hartinger CG, Timerbaev AR, Keppler BK, Jarosz M (2006) Platinum group metallodrug-protein binding studies by capillary electrophoresis - inductively coupled plasma-mass spectrometry a further insight into the reactivity... 

FIGURE 3.33 Ultrafiltration apparatus for drag-protein binding studies. 

For the measurement of chiral interactions, e.g. in the course of drug-protein binding studies [4] and other thermodynamic measurements [5]. 

Protein type CSPs have to be considered as important and widely used tools for enantiomer separations (Table 9.9) and for analyzing stereoselective phenomena of biological significance, in particular for stereoselective drug-protein binding studies. The latter application, however, is beyond the scope of this report and the reader is referred to recent articles on this topic [4,223-232]. 

The concentration of AGP in the plasma of healthy volunteers correlated significantly with the binding ratio (bound/free) of the enantiomers, as well as the racemic form, of methadone. The average free fractions for +)- and (—)-methadone in plasma were 0.100 and 0.142, respectively (97). Two variants of AGP, orosomucoids 2A and FI, were also found to play important determining roles in the binding of methadone enantiomers (97). The authors of the study point out that the levels of AGP variants should be considered in protein binding studies. 

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