Protected peptide fragments analysis

TLC Analysis of protected peptide fragments is described in Chapter 9, Protocol 2. Typically, the maximal concentration of peptide may be contained in the first wash or in one of the later washes, depending on the buffering capacity of the amide bonds and other functional groups present. 

DNS derivatives of amino acids and peptides are used for the protection of amino groups, separation by TLC and fluorimetric analysis. DNS derivatives can be also used for MS identification and have been employed for the confirmation of a large number of biogenic amines [209]. The molecular ions of these derivatives are usually intense and are often accompanied by an ion at m/e 170 or 171 as the key fragment. BNS derivatives are also useful for MS identification of amines of biological interest [82,83]. 

---