Pronuclei assembly

Surf Clam. Longo et al. (1994) have examined the Spisula lamin L67 in male pronuclei assembled in vitro. Nuclei are fixed in 3% paraformaldehyde in PBS for 1 hr and washed in PBS as described earlier. Fixed and washed nuclei are applied to a poly(L-lysine)-coated cover slip. Nuclei are reacted for 30 min with a 1 100 dilution of the anti-Spisula L67 Pab227 antibody. Cover slips are washed extensively in PBS, incubated for 30 min in a 1 20 dilution of FITC-conjugated antirabbit antibody, washed in PBS, and mounted in glycerol. 

The reassembly of the NE at the end of mitosis is the best recognized example of NE assembly in eukaryotes. Another, perhaps less appreciated, example of NE assembly in metazoans is that which occurs around sperm chromatin during the formation of the male pronucleus after fertilization (for review, see Longo... 

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