Promoters chimeric

Figure 1. Schematic map of the genomic DNA region of hydA and of chimeric hydA promoter constructs.

The presence of the chimeric construct in each ars-transformant was verified by PCR using an upstream oligonucleotide that hybridizes to the hydA promoter fragment and a downstream oligonucleotide that hybridizes to the ars gene asindicated with black arrows at the bottom. 

Omirulleh, S Abraham, M., Golovkin, M., Stefanov, 1., Karabaev, M.K., Mustardy, L., Morocz, S., and Dudits, D. (1993). Activity of a chimeric promoter with the doubled CaMV 35S enhancer element in protoplast-derived cells and transgenic plants in maize. Plant Mol. Biol. 21(3) 415-428. 

Figure 4.4 Nucleolar localized anti-HIV-1 ribozyme. The anti-HIV-1 ribozyme was inserted within the apical loop of the small nucleolar RNA U16. The chimeric U16-ribozyme was placed behind the Pol III U6 snRNA promoter. Transcripts of the chimeric RNA localize to the nucleolus, as shown by the in situ hybridization and co-localization with the small nucleolar RNA U3. The in situ hybrids were performed in transfected 293 cells. The ribozyme probe was fluorescein labeled (green), whereas the U3 probe was labeled with Cy3 (red). Reproduced with permission from Michienzi et al. (2000). (see Color Plate 3)...

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