Prokaryotic culture systems

In this chapter, novel aspects of the manufacturing procedures of monoclonal antibodies from a process engineering point of view wiU be highhghted. The manufacture of monoclonal antibodies in mammalian cell culture requires certain technologies and capacities, which will be described within the first section of the chapter. Technical hurdles finked to these still-expensive procedures and aimed towards the development for more economical eukaryotic or even prokaryotic manufacturing systems will be highlighted in the second section. 

While whole antibodies are currently produced in expensive mammahan cell culture systems, for some appHcations the expression of antibody fragments in prokaryotes, yeast or filamentous fungi is an alternative. Tissue penetration is facilitated, and clearance from the blood or kidney is faster using antibody fragments. In addition, PEGylation allows the modification of the half-Hfe, which is another advantage of antibody fragments (see also Part VI, Chapter 2). 

Use of Prokaryotic and Eukaryotic Culture Systems for Examining Biological Activity of Food Constituents... 

The new metabolite [l-(2 -methylpropoxy)-2-hydroxy-2-methylpropoxyjbutane 345 was isolated from the cell-free culture supernatant of the marine bacterium Vibrio angustum S14 eis part of studies investigating the role of chemical signals in prokaryote-prokaryote and prokaryote-eukaryote interactions. Compound 345 induced the acylated homoserine lactone (AHL) reporter system in Agrobacterium tumefaciens and bioluminescence in Vibrio harveyi. 

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