Progesterone challenge

If the patient demonstrates withdrawal bleeding after the progesterone challenge test, this indicates that the ovaries are producing sufficient estrogen to cause endometrial proliferation and there is no anatomical obstruction. Most of these women have a history of oral contraceptive use, stress, weight loss, or excessive exercise. 

The analysis of steroids is a challenging task. GC-MS has frequently been applied, but requires analyte derivatization [1]. Therefore, over the years most LC-MS interfaces have been tested or applied in the analysis of steroids. As early as 1981, Henion [2] demonstrated the analysis of dexamethasone and cortisone with micro-LC coupled to a capillary-inlet interface. Van der Greef et al. [3] described the quantitation of progesterone in serum using isotope dilution MS in anunonium Cl mode using the moving-belt interface. Henion and coworkers [4] described... 

In the bovine estrus synchronization therapy, as was mentioned previously, supraluteal levels of progesterone (>lng ml ) are obtained a short time after the insertion of silicone devices due to a quick absorption of the hormone through the vaginal wall, followed by a complex but fast distribution to the circulatory system. One challenge in developing... 

---