Prochiral protons, assignment

Nucleosides stereospecifically deuteriated at C-5 have been prepared in order to assign the signals for the prochiral protons at this position. In all cases, the more deshielded proton occupied the pro-R position. The data was used to determine the rotamer population about the C(4 ) - C(5 ) bond based... 

High resolution NMR studies, in combination with molecular mechanics, were performed to unequivocally assign the prochiral P-protons for the phenylalanine sidechain and to define the shape of the sweet receptor [49], The previous assignment [48] of the NMR signals for the P-protons of phenylalanine was shown to be in error. 

The side chain dihedral angle Xi is important for high-resolution definition of protein structures as it determines the angle at which each amino acid side chain branches out from the protein backbone. Moreover, in combination with certain types of NOEs, it can allow stereospecific assignment of prochiral /3-methylene protons, which improves the precision of NMR structures by obviating the need to include pseudoatom distance corrections.140-142... 

For labeled proteins, the 3D FINFIB experiment147 has become the most popular approach for determining Xi and for providing stereospecific assignment of prochiral /3-methylene protons. In contrast with the... 

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