Process gel filtration

Regardless of their method of fabrication, most liposome preparations need to be further classified and purified before use. To remove excess aqueous components that were not encapsulated during the vesicle formation process, gel filtration using a column of Sephadex G-50 or dialysis can be employed. To fractionate the liposome population according to size, gel filtration using a column of Sepharose 2B or 4B should be done. 

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