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Primers sticky

Preparation of Blunt-Ended Amplification Fragments. Kinased primers are used in the PCR, and the ends of PCR fragments are flushed with E. coli DNA polymerase I and dNTPs to increase the efficiency of ligation. The fragments are then purified as described for the sticky ended fragments. [Pg.437]

In most cases HTV rubbers are bonded to solid substrates using undercuts or primers. Self-adhesive HTV rubbers work in the same way as their LR counterparts. However, moulding is more critical because of substantially longer curing times which can lead to demoulding problems, as mould stickiness increases dramatically with curing time. For such purposes the processor might need to prime his moulds with a special PTFE primer. It should be noted that self-adhesive HTV sticks to steel when moulded ... [Pg.299]

The vehicle of quality paints, especially wall paints and primers, are sticky if used in high concentration because they are well-polymerized resins. This high degree of polymerization makes the product viscous and also improves adhesion, toughness, and chemical and weathering resistanee. In addition, polymerized resins prevent striking in due to their resistanee to substrate suction. These well-polymerized film formers must be diluted with solvents before application. However, the lower solid level resulting from this... [Pg.56]


See other pages where Primers sticky is mentioned: [Pg.111]    [Pg.320]    [Pg.454]    [Pg.83]    [Pg.84]    [Pg.84]    [Pg.424]    [Pg.375]    [Pg.320]    [Pg.201]    [Pg.209]    [Pg.544]    [Pg.360]    [Pg.40]    [Pg.861]    [Pg.384]    [Pg.385]    [Pg.55]   
See also in sourсe #XX -- [ Pg.183 ]




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