Preparative HPLC adsorption isotherms

According to the equilibrium dispersive model and adsorption isotherm models the equilibrium data and isotherm model parameters can be calculated and compared with experimental data. It was found that frontal analysis is an effective technique for the study of multicomponent adsorption equilibria [92], As has been previously mentioned, pure pigments and dyes are generally not necessary, therefore, frontal analysis and preparative RP-HPLC techniques have not been frequently applied in their analysis. 

The aim of this chapter is to highlight current developments in these various fields of preparative HPLC, with particular emphasis on applications that have been developed at Chemical Analytical Development at Novartis Pharma AG. Drug substance purifications from biological and synthetic sources are presented, along with the separation of chiral and/or achiral molecules on chiral stationary phases and typical isolations of by-products. Special attention is given to the determination of adsorption isotherms and their interplay with respect to the layout of chromatographic processes as well as the choice of... 

Adsorption isotherms and Henry constants are only really of significance in preparative enantioselective HPLC and simulated moving bed (SMB) applications. 

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