Preferred Acidolysis Procedure

Preferred Acidolysis Procedure 6.1.4.1 Preparation of the Acidolysis Reagent... 

The rate of derivatization of different compounds varies when trimethylsilyl derivatives are prepared (Lundquist and Kirk 1971). Another complication arises from the fact that enolization and subsequent formation of enol derivatives occur during the derivatization treatment (Lundquist and Kirk 1971). Ketol la (Fig. 6.1.1), for example, has been shown to give rise to three different derivatives (Fig. 6.1.3) (Lapierre et al. 1983a). One approach for overcoming these difficulties consists of derivatization under mild conditions in combination with a gas chromatographic study of the rate of derivatization (Lundquist and Kirk 1971, Pometto and Crawford 1985). A second solution to this problem consists of performing the silylation under conditions that secure complete derivatization and analysis of all types of derivatives formed (Lapierre et al. 1983a). The latter principle has been adopted in the preferred acidolysis procedure. 

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