Prediction of Initial Velocity Patterns

The complete analysis of the primary and the secondary double reciprocal plots is often not necessary to identify the mechanism the mechanism can frequently be determined from the variation of apparent Michaelis constants and maximal rates with the concentration of the fixed substrate (Cleland, 1970, 1977). 

The apparent Michaelis constant is the slope of a primary reciprocal plot (of l/Uo versus 1/[substrate]) divided by the vertical intercept, and the apparent maximal velocity is the reciprocal of this intercept. 

We shall distinguish three types of initial velocity patterns  

The equilibrium ordered patterns vary depending on which substrate is varied, whereas the former two patterns do not. 

For example, for the Ordered bisubstrate mechanism, the apparent Michaelis constants for A and B are 

---