Prediction of Binding Rates

At the other end of the spectrum, conformational rearrangement is rate-limiting (i.e., kc k o)/ and 

Note thatko/k-D is the equilibrium constant for forming the transient complex. 

FIGURE 4.4 The spectrum of protein binding rates. The different regimes are indicated by arrows. For protein complexes in the electrostatically-enhanced regime are shown. Adapted from Alsallaq and Zhou [19]. 

To go beyond the basal rate km and reach rates like 10 to 10 s as 

From BD simulations [54,73,91,92] and analytical results [57,93], it was discovered that the diffusion-controlled rate can be accurately approximated as 

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