Practical Aspects and Applications in Bioanalysis

One critical feature of mass spectrometry when combined with chromatographic or electrophoretic separation techniques remains the duty cycle of the mass analyzer. A conventional LC chromatographic peak lasts about 10 s, which is sufficient to perform various MS and MS/MS experiments on various types of instruments. In the case of fast LC, the peak width can be in the range 1-2 s which is too fast for most mass analyzers except for TOF mass spectrometers. 

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For those readers who are not yet familiar with mass spectrometry, the introduction provides an explanation of the basics of mass spectrometry and its instrumentation as well as practical aspects and applications in bioanalysis. Next, a block of three chapters shows different affinity selection procedures suitable to identify hits from combinatorial compound libraries. This subject, being metaphorically speaking a search for a needle in a haystack, is of outstanding relevance for big pharma . The techniques described here offer real high throughput capabilities and are implemented already in the routine industrial screening... 

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