POSFALF Mode

STRATEGY FOR USE OF SMART ROUTES TO PREPARE LABEL-FREE APTASENSORS 

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POSOALF Mode PFSOALF Mode POSFALF Mode PFSFALF Mode... 

In fact, aptamers selected capable of reporting domains are limited. Another series of fluorescent aptasensors take different routes and serve as complementary ways to make the PFSFALF more easily prepared and available for wider applications. This type of sensor is more similar to the POSFALF mode and can also be divided into two parts the aptamer beacon approach and the duplex-to-complex switching approach. 

Figure 12.11 Two approaches to designing POSFALF fluorescent aptasensors. The aptamer beacon approach contains two modes of signal-on (Al) (Yamamoto and Kumar, 2000 Hamaguchi et al., 2001) and signal-off/FRET (A2) (Li et al., 2002 Ueyama et al., 2002). The duplex-to-complex switching approach contains two signal-on modes B1 (Rupcich et al., 2005 Elowe et al., 2006) and B2 (Nutiu and Li, 2003).

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