Porous anodic alumina layer chip

Figure 7.6 Experimental setup and construction of LSPR and interferometry-based label-free optical aptasensor with a porous anodic alumina layer chip.

Here we demonstrate the combination of LSPR with interferometry using a gold-coated porous anodic alumina (PAA) layer chip for the detection of aptamer-protein interactions (Figure 7.6). Recently, we have employed this device for the detection of DNA hybridization (Kim et al., 2007). Gold deposition on the surface of the PAA layer chip enables the formation of a novel nanomaterial, which we called gold-capped oxide nanostructures. One of... 

Figure 7.7 (A) AFM analysis of a porous anodic alumina (PAA) layer chip surface...

Figure 7.7 (A) AFM analysis of a porous anodic alumina (PAA) layer chip surface after Au deposition. (B) Detection of a sandwich-type binding assay between the aptamers and thrombin using Au-capped oxide nanostructures on the PAA layer chip. (C) Relative reflected intensity characteristics of (black line) a bare PAA layer chip, 10 p.M aptamer I immobilized on a gold-deposited PAA layer surface (red line), the binding reaction between 10 p.M aptamer I and 1 p.M thrombin on the chip surface (blue line), after the binding reaction between aptamer I/thrombin complex and aptamer II (green line). (See text for full description.)...

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