Porcine FSH

Follicle-Stimulating hormone-releasing hormone (FSH-RH). FSH-RH was discussed at this meeting by Dr. McCann and Dr. Jutisz. Our preparations of porcine and bovine FSH-RH seem to be considerably more potent than their preparations of ovine FSH-RH and are also free of LH> H. A complete separation of FSH-RH from LH-RH was achieved by preparative column electrophoresis (3, 18, 19). Thus fractions which release LH and have no effect on FSH secretion and conversely fractions which release FSH and have no effect on IM secretion. Highly purified FSH-RH at doses of 4-10 nanog depletes pituitary FSH content in castrated male rats pretreated with testosterone (3, 19). FSH-RH will also deplete pituitary FSH in normal male rats (3, 19). Some amines such as putrescine are also capable of depleting pituitary FSH content in vivo (20). Mittler and Meites (36) were the first to demonstrate the stimulatory effect of rat hypothalamic extracts on the release of FSH in vitro. e have shown by in vitro experiments that purified porcine FSH-RH stimulates the release of FSH by a direct action on pituitary tissue (21). Thus, addition of partially purified FSH-RH, but not of histamine, greatly enhanced the release of FSH. 

Porcine FSH has been found to contain 2.1% of sialic acid." ... 

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