Polysaccharides osmotic pressure method

By methylation end-group assay. 1 Number-average degree of polymerization of methylated polysaccharide by id (isothermal distillation) or op (osmotic pressure) methods. c Viscosity-average degree of polymerization. 

Methods for the determination of the molecular weight of a polysaccharide include gel-filtration (Andrews, 1970), ultracentrifugation, light scattering, and osmotic pressure determinations (Banks and Greenwood,... 

In parallel, another important (although less direct) technique for measuring forces between macromolecules or lipid bilayers was developed, namely, the osmotic stress method [39-41]. A dispersion of vesicles or macromolecules is equilibrated with a reservoir solution containing water and other small solutes, which can freely exchange with the dispersion phase. The reservoir also contains a polymer that cannot diffuse into the dispersion. The polymer concentration determines the osmotic stress acting on the dispersion. The spacing between the macromolecules or vesicles is measured by X-ray diffraction (XRD). In this way, one obtains pressure-versus-distance curves. The osmotic stress method is used to measure interactions between lipid bilayers, DNA, polysaccharides, proteins, and other macromolecules [36]. It was particularly successful in studying the hydration... 

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