Polysaccharides matrices coupling

Sanderson and Wilson [16] described a coupling procedure for polysaccharide matrices based on periodate oxidation. The general course of the reaction follows that of polysaccharide oxidation by periodate as described by Guthrie [17]. In the matrix activation step, the polysac-... 

Figure 4.62 One-electron oxidation of feruloyl groups. The radicals can couple at all possible spin sites, although (3-04 is the most frequent, g Lignin, the matrix material of plant cells, is an oxidative polymer of three related alcohols, i-coumaryl, coniferyl and sinapyl alcohol, formed by coupling of radicals at all possible spin sites. The feruloyl side-chains of pectin and hemicellulose can participate in this polymer formation, generating covalent links between polysaccharides and lignin.

One of the most important factors in the area of affinity chromatography is the development of solid supports. A correct choice of a solid support and the covalent coupling between the matrix and the affinity ligand may be essential for the success of the desired separation. A solid support may even constitute an affinity ligand itself, for example, polysaccharides for some lectins [63]. 

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