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Polypropylene trypsin

Fig. 1. Schematic for the design of the tissue incubation chamber. The outer chamber is made from Perspex and sits on a magnetic stirrer The top has a circular hole cut to the diameter of the polypropylene tube containing the PIPES/trypsin solution whereas the base has a small podium machined to accommodate the base of the tube Oxygen is continuously supplied by a tube attached to a loose-fitting cap. Water maintained at the required incubation temperature is pumped in the direction shown... Fig. 1. Schematic for the design of the tissue incubation chamber. The outer chamber is made from Perspex and sits on a magnetic stirrer The top has a circular hole cut to the diameter of the polypropylene tube containing the PIPES/trypsin solution whereas the base has a small podium machined to accommodate the base of the tube Oxygen is continuously supplied by a tube attached to a loose-fitting cap. Water maintained at the required incubation temperature is pumped in the direction shown...
Transfer 25 mL of PIPES solution, presaturated with 100% oxygen, into a polypropylene tube and add 5000 U/mL (0.07%, w/v) of trypsin. Place the tube in... [Pg.27]

Remove the polypropylene tube filled with the PIPES/trypsin solution from the incubation chamber and, using a wide-tipped Pasteur pipet, transfer the 2 x 2 nun pieces of tissue into the tube. [Pg.28]

See other pages where Polypropylene trypsin is mentioned: [Pg.522]    [Pg.167]    [Pg.573]    [Pg.7]    [Pg.333]    [Pg.390]    [Pg.808]   
See also in sourсe #XX -- [ Pg.713 ]



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