Polyneuridine Aldehyde Esterase PNAE

PNAE was the first enzyme detected in the ajmahne pathway. Detection and prehminary characterization were first reported in 1983. This enzyme turned out to be an extraordinary highly substrate selective protein which has been reported several times. In order to achieve a comprehensive purification of PNAE, an HPLC-based assay was developed for 

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Figure 14 Catalytic function of polyneuridine aldehyde esterase (PNAE), delivering the pathway intermediate 49 through the unstable alkaloid polyneuridine aldehydoacid (64). Taken from Ref. 106.

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