Polymer brushes assay

Keywords Assay Bioactive surface Cell adhesion Cell chip Hydrogel Microengineering Micropattern Peptide Polymer brush Protein adsorption... 

Thermally responsive polymers, such as poly( V-isopropyl acrylamide) (NI-PAm), have also been studied extensively for applications related to those previously discussed [112], De las Heras et al. described the synthesis and patterning of NIPAm brushes on SAMs and their subsequent performance during temperature-dependent adhesion assays of BSA and Streptococcus mutans (Fig. 7). The authors employed p.CP to pattern features of hydrophobic hexadecanethiol and backfilled the surface with an initiator-functionalized alkanethiol. Polymer brushes were grown via surface-initiated atom transfer radical polymerization (ATRP). FITC-BSA was then... 

Figure 4.14 Polymer brushes with enzymatic activity. (A) Enzymes are physisorbed in polymer brushes with different character (e.g, neutral or charged). (B) The activity of the enzymes such as alkaline phosphatase and horseradish peroxidase (rightj is assayed with colorimetry.

Advanced analytical methods enable an in-depth study of chemical and morphological characteristics of micro- and nanografted polymer materials. Often, the combination of several techniques is necessary to obtain a detailed picture. Investigations of simplified model systems such as nonstructured grafted surfaces, brushes produced on nonpoly-meric substrates, or selectively derivatized or stained samples are needed for comparison or as standards. Furthermore, the adaptation of biochemical and chemical assay techniques is useful to complete the picture of the produced structures and their functionalities. 

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