Polyamide nucleic acids synthesis

Will, D. W., Breipohl, G., Langner, D., et al. (1995) The synthesis of polyamide nucleic acids using a novel monomethoxytrityl protecting-group strategy. Tetrahedron, 51, 12069-12082. 

Fig 19 Synthesis of polyamide nucleic acids (PNA). (A) Generation of a monomeric thyminyl building block. (B) Coupling of the monomer to the solid support. (C) Merrifield synthesis using Boc-strategy. (Boc = tert-butoxy carbonyl, Pfp = pentafluoro phenyl, S = 4-methylbenzhydrylamine resin)... 

Just as the Merrifield synthesis in Chapter 27 (Section 27.4.2) is used to prepare polyamides by using a polymer bead to anchor the growing polyamide chain, there is a method for making nucleic acids using a pol3rmer support. Of the several polymer supports used, styrene or copolymers of styrene are used most of the time. If polystyrene (see Chapter 10, Section 10.8.3) is used as a support, a chemical reaction is required to attach the first nucleotide. This is accomplished by preparing a polystyrene derivative in which the benzene rings are functionalized. 

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