Polyacrylamide hydrazine, immobilization

The glutaraldehyde and hydrazine reactions are used for matrices having amide groups such as polyacrylamide. Glutaraldehyde and hydrazine react with the polymer, and the enzyme or protein is readily bound to the treated polymer. The mechanism of the reactions involved in the activation and coupling are not well understood. In general, insoluble supports such as polyacrylamide, which tend to swell in water, form immobilized proteins or enzymes when mixed with a solution and treated with... 

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