Poly boronate chromatography

Boronic Acids as Binding Sites. Poly(vinylphenylboronic acids) are commercially available and can be used in an alkaline aqueous medium for the purification and chromatography of diol-containing compounds (64-66). In organic solvents they usually form trigonal borondiester bonds (see Equation 1 and 2). [Pg.203]

The Endogenous Mono- and Poly(ADPR) Nonhistone Proteins of Yoshida Hepatoma Cells can be Isolated by Aminophenyl Boronic Acid Chromatography... [Pg.264]

Fig. 2. Covalent attachment of poly(ADPR) to the nonhistone proteins being retained by boronate agarose chromatography. The fraction of nonhistone proteins being retained by the boronate resin was separated by sodium dodecyl sulfate electrophoresis (cf. Fig. 1). The stained gel was sliced and poly(ADPR) determined in individual fractions utilizing a specific radioimmunoassay [1]...

$Fig. 2. Covalent attachment of poly(ADPR) to the nonhistone proteins being retained by boronate agarose chromatography. The fraction of nonhistone proteins being retained by the boronate resin was separated by sodium dodecyl sulfate electrophoresis (cf. Fig. 1). The stained gel was sliced and poly(ADPR) determined in individual fractions utilizing a specific radioimmunoassay [1]...$

Poly(ADP-ribose) modified proteins were isolated from E63 cells at different stages of myogenesis. Isolation and analysis of these proteins was performed essentially as described by Adamietz et al. [16] using boronate cellulose chromatography and per-... [Pg.441]

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