Pollutant binding

Tatsumi K, A Freyer, RD Minard, J-M Bollag (1994) Enzyme-mediated coupling of 3,4-dichloroaniline and ferulic acid a model for pollutant binding to humic materials. Environ Sci Technol 28 210-215. 

Landrum, R.F., Nihart, S.R., Edie, B J., Gardner, W.S. (1984) Reverse-phase separation method for determining pollutant binding to Aldrich humic acid and dissolved organic carbon of natural waters. Environ. Sci. Technol. 18, 187-192. 

Fig. 10.3. Different anal3ttical approaches based on bacterial systems, (a) In the presence of toxic substances, the protein production is suppressed as a consequence of cell death or inhibition of its metabohsm. (b) The reporter gene is controlled by a promoter that will respond to a specific pollutant. In this case, the pollutant binds specifically to the regulatory protein being released from the promoter and allowing the transcription of the reporter gene, (c) The reporter gene is expressed under the control of a promoter that is responsive to a wide range of conditions that are potentially harmful to the cells, thus the presence of a very broad spectrum of pollutants induces the expression of the reporter gene.

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