Plots for Michaelis-Menten Expression

Because straight-line plots are easier to evaluate than curves, it is convenient to reformulate Equation (6.4) to yield straight-line plots. Two such reformulations can be performed. The Lineweaver-Burk plot is a double-reciprocal plot, obtained by taking reciprocals of both sides of Equation (6.4) and rearranging  

According to Equation (6.6), if the system obeys Michaelis-Menten kinetics, a plot of 1 /v versus 1 /[S] is a straight line having a slope of Km/ Vmax and an intercept of 1/Vmax on the 1/v axis. Furthermore, the intercept on the 1/[S] axis (occurring when 1/v = 0) is —1/ m- This is seen by setting 1 /v equal to zero. If 

Two linear plots for the same data obtained for an enzyme-catalyzed reaction that obeys Michaelis-Menten kinetics. 

The Eadie-Hofstee plot represents an improvement over the Lineweaver-Burk plot in that the experimental points are usually more equally spaced. We obtain the equation for the Eadie-Hofstee plot by rearranging Equation (6.4)  

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