Ploidy polyploid

Two approaches are currently known for obtaining transformed root cultures of different ploidy level transformation of autopolyploid intact plants and in vitro polyploidization of transformed root cultures obtained from diploid intact plants. 

The approach of in vitro polyploidization of transformed root cultures obtained from diploid intact plants is presented schematically on Fig. 7.6. Most often, for in vitro polyploidization of transformed root culmres, colchicine 31 and oryzalin 32 are used in concentrations of 0.002-0.005% and 0.0002-0.0005%, respectively. Our experience shows that about 8-10% of the newly obtained lines are of different ploidy than the source line. 

---