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PLIMSTEX in Biologically Relevant Media and High Ionic Strength

2 PLIMSTEX in Biologically Relevant Media and High Ionic Strength [Pg.352]

Apo-IFABP and Oleate A Protein-Small Organic Molecule Interaction [Pg.353]

Intestinal fatty acid-binding protein (lEABP) belongs to a family of 15 IcDa, predominately y -sheet proteins that bind a diverse group of polar lipids [49]. lEABP [Pg.353]

The PLIMSTEX curve for 0.3 pM WT-IFABP titrated with potassium oleate fits well with a 1 1 binding model [22, 24], The K and ADi (difference between the average deuterium level of one-ligand-bound protein and that of apo protein) for WT-IFABP are (2.6 + 0.6) x lO and 13.8 + 0.7 (Table 11.1), respectively, indicating that a strong interaction between oleate and WT-IFABP occurs with protection of 14 backbone amide hydrogens. [Pg.354]

These results demonstrate that the disruption of the D34-R126 ion pair in IFABP causes the D34A mutant to bind additional oleates (at least three) with lower binding constants than for WT-IFABP binding the first oleate. [Pg.354]




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And ionic strength

Biological media

Biological relevance

High ionic strength

High medium

Ionic media

Ionic strength

PLIMSTEX

Strength media

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