Platelets, density gradient centrifugation

In order to determine the blood compatibility of the plasma treated materials, platelet adhesion studies were conducted since platelet adhesion is one of the most important steps during blood coagulation on artificial surfaces. Blood samples were drawn from healthy canines (adult mongrel dog) which were not on medication. Platelet-rich plasma was obtained and the platelets were washed by albumin density gradient centrifugation in Hepes-Tyrodes buffer solution of pH 7.3 by a modification of Walsh and Griffin s... 

---