Plate height size exclusion

It is useful to choose a solute which is really eluted by a size-exclusion process, without adsorption or any additional interaction phenomena which might modify the shape of the peak. The most trivial method is to analyze the shape of a low-MW pure substance. This is usually used to determine the number of theoretical plates, N = V,lcrf, where V, is the retention volume (volume at peak top) and a is the standard deviation, cr can be computed from the weighing of each data point of the peak or can be estimated from the width at 10% maximum height (cr = Wo,/4.3). 

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