Plasmid-borne reporter genes

Figure 3. Role of enhancers and histone acetylation in regulating gene expression. A plasmid-borne reporter gene was either driven by the tk promoter (Promoter) or was coupled with the FI 01 enhancer (Enhancer). In some cases the embryos were also cultured in the presence of butyrate, which induces histone hyperacetylation (Promoter-FButyrate, Enhancer-FButyrate). Either male pronucleus (Male PN), female pronucleus (Female PN), or zygotic nucleus (two-cell) was injected. When one-cell embryos were injected, they were cultured in the presence of aphidicolin to a time that corresponded to the mid to late Iwo-cell stage. The data are expressed relative to the amount of luciferase activity detected in the male pronucleus of an S-phase-arrested, one-cell embryo and were taken from Wiekowski et al., 1993.

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