Plan apochromats

Neofluar objectives are chromatically better corrected than the achromatic objectives (fluorite), but worse than the plan-apochromat objectives. The contrast of the neofluar objectives is however larger than with the plan-apochromat objectives. 

Fig. 5. Optical sectioning of rhodamine phalloidin-stained F-actin in a neutrophil migrating through a 5-pm pore of a polycarbonate membrane. The neutrophil migration is stimulated in response to 10 M Af-formytmethionyl-leucy 1-phenylalanine. (A), (B), and (C) correspond to O.S-pm optical sections indicated as sections A, B, and C, respectively, in Fig. 4. The bar represents 10 pm. The images were collected with a Nikon Microphot FX microscope (x60 Plan-apochromat lens, numerical aperture, 1.6) connected to a Bio-Rad MRC600 laser-scanning confocal system.

Analyze the distribution of fluorescence in live unfixed cells on a Zeiss Axiovert 200 M fluorescence microscope with 63 x Plan-Apochromat objective, an Axio-Cam MRm camera, and Axiovision software. 

Perform the experiments with a 60x/1.45 Plan-Apochromat oil objective. 

Plan Apo (plan apochromatic) Corrected for spherical aberration and spectral continuity. These objectives may contain up to 15 separate lenses, as reflected in their price. For black-and-white photography and under the correct lighting conditions, a Plan lens may equal a Plan Apo lens in resolution. However, for color photomicrographs, the latter is preferred. 

The Bio-Rad scanning system is attached to a TE300 Microscope (Nikon Surrey, UK), with a 60 X 1.2 NA plan apochromat water immersion lens (Nikon). For in situ 3D imaging the standard configuration is combined with a 1.5 x digital zoom... 

Generally optical lenses are very well corrected they have been able to obtain resolutions very close to the theoretical diffraction limit for a century or more. However, full correction of chromatic aberration, spherical aberration, and field curvature to give a Plan Apochromat will be expensive. In contrast, lenses for electron microscopy are generally... 

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