Phytosterols sample preparation

Furthermore, using UHPLC-MS in VOO phytosterols analysis led to a remarkable reduction in the analysis time (both in the sample preparation step and in the phytosterols separation). The separation was achieved in 5 min, time in which 14 phytosterol compounds were identified (Figure 9.8). Thereby, the analysis time using UHPLC-MS is four to six times faster than those of HPLC-MS methods [22,82,90], where the time required is between 25 and 30 min. The comparison is even more... 

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