Physiological state of bacteria

Attachment of E. coli to HeLa cells. Attachment at different levels of inoculation (10 -106 CFU/mL) and physiological state of bacteria was investigated as described above. Typical results are presented in Table 1. There was no significant difference in attachment of actively growing and starved cells to the monolayer of epithelial cells. The difference in attachment between strains did not correlate with pathogenicity and was not statistically different for all investigated strains. 

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