Phycobiliprotein absorption spectra

The absorption spectrum of the OECC purified from P.la-minosum grown in Fe-enriched medium showed main peaks at 674, 492, 435 and 414 nm (Fig. 1). The last step of purification, i.e. precipitation with polyethylene glycol, eliminated considerable amounts of phycobiliproteins, absorbing between 600-650 nm, which usually contaminate this kind of preparation. The bands at 674 and 435 nm are due to Chi and that at 414 nm is mainly due to the cytochrome b-559. The carotenoids absorb in the 492 nm region. The OECC preparation showed an O2-evolution rate of 2,000 /wnol. mg Chl . hT, ... 

Biosensor in Table 1-8 is a gel entrapping biomolecules, such as proteins, which react with molecules to be analyzed, resulting in a change in spectra of optical absorption or fluorescence (Dave, 1994). Phycobiliprotein-doped gel (Chen, 1996), for instance, presents a sensor based on the change in fluorescence spectrum. Availability of carbon-enzyme hybrid electrodes is discussed with glucose biosensor as a test case (Sampath, 1996). 

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