Photochemical reagents fluorescent

Stern-volmer kinetic relationships This term apphes broadly to variations of quantum yields of photophysical processes e.g., fluorescence or phosphorescence) or photochemical reaction (usually reaction quantum yield) with the concentration of a given reagent which may be a substrate or a quencher. In the simplest case, a plot of (or /M for emission) vs. concentration of quencher, [Q], is linear, obeying the equation... 

During sample preparation reagents such as the extraction water were examined for contamination by UV-Vls, fluorescence spectroscopy, and excited state absorption after laser excitation. Also the samples were checked for changes In the above spectral characteristic before storage and Immediately prior to analysis. If there were significant changes the sample was dlsgarded as unsuitable for futher photochemical analysis. 

---