Photoaffinity labeling examples

Figure 8.19 Examples of photoaffinity labels. (A) An aryl azide and (B) a benzophenone.

Specific Examples of the Application of Photoaffinity Labeling in Signaling Pathways... 

Photochemically reactive molecules have often been used as labels for specific sites in proteins and nucleic acids. Psoralen derivatives serve as relatively nonspecific photochemically activated crosslinking agents for DNA and double-stranded RNA.195 Aryl azides are converted by light to aryl nitrenes, which react in a variety of ways including insertion into C-H bonds (Eq. 23-27).200 201 In some cases UV irradiation can be used to join natural substrates to enzymes or hormones to receptors. For example, progesterone, testosterone, and other steroids have been used for direct photoaffinity labeling of their receptors.202 Synthetic benzophenones have also been used widely as photoactivated probes.203... 

Some examples of photoaffinity labeling and related experiments... 

To induce the reader to proceed further, the remainder of this short chapter gives a brief account of some applications of photogenerated reagents. Five classes of photoaffinity labeling experiments are described followed by three examples of the related methods that are discussed in the later chapters of this book. 

Fig. II Examples of MS-ffiendly photoreactive compounds used in mixed photoaffinity labeling. CsA cyclosporin A...

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