Photoaffinity labeling control experiments

Several controls related to those required in photoaffinity labeling experiments with radiolabeled ligands (see Section 4.7) must also be performed in studies of photoinactivation. In particular inactivation should not occur on irradiation in the absence of the photoaffinity label, and the receptor site should be protected against photoinactivation if it is first blocked with a photochemically inert molecule. 

Evidence for Specificity and the Site of the Reaction. The specificity of the photoaffinity labeling reaction was checked by the control experi-... 

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