Phosphotrieste oligodeoxynucleotides

There are three widely used chemistries for oligodeoxynucleotide synthesis, and all have been successfully transferred onto SP. The first is the so-called phosphotriester approach (71), which is shown in Fig. 2.9. In this method a 5 -protected nucleoside... 

Andrus A, Beaucage SL. 2-Mercaptobenzothiazole An improved reagent for the removal of methylphosphate protecting groups from oligodeoxynucleotide phosphotriesters. Tetrahedron Lett 29 5479-5482, 1988. 

Synthesis of 01igodeo iQrribonucleotides by the Phosphotriester Method. l-(Mesitylene-2-sulfonyl)-3-nitro-l,2,4-triazole-(MSNT) has been widely used as a condensing agent for the synthesis of oligodeoxynucleotides by the phosphotriester method. ... 

As in solution syntheses, two types of strategies have been used for oligodeoxynucleotide synthesis. These are the diester and the triester approaches. The earliest attempts by Letsinger and co-workers employed the triester approach. This is illustrated in Schemes 5-3 and 5-4, X = H). Most of the recent work has employed the diester approach. The advemtage in this particular approach is that the phosphorylating reagents are more reactive than those leading to phosphotriesters. Most workers... 

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