Phosphoryl transferases design

Independently, Umezawa and coworkers and Brzezinska and Davies" found another enzyme, designated kanamycin phosphate transferase II or kanamycin-neomydn phosphate transferase II, in E. coli canning R factor, and it was found by the former authors that this enz)one also catalyzes the transfer of a phosphate group from adenosine 5 -triphos-phate to the 3 -hydroxyl group of the kanamycins, paromamine, neamine, neomycin, ribostamydn, and the butirosins. Therefore, kanamycin-neomycin phosphate transferase II differs from I in the following points II phosphorylates butirosin, but not lividomydn, whereas I phospho-rylates the latter, but not the former. 

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